anti oas2  (Cell Signaling Technology Inc)

Journal: Molecular Pharmacology

Article Title: Transient receptor potential ankyrin 1 promotes the expression of interferon-stimulated antiviral genes in human A549 lung epithelial cells

doi: 10.1016/j.molpha.2025.100098

Figure Lengend Snippet: The effect of TRPA1 antagonists on the mRNA expression of the IFN-stimulated antiviral genes MX1, OAS2, and DDX58 . A549 cells were cultured with IFN beta or IFN alfa-2a (both 10 ng/mL) in the presence or absence of the TRPA1 antagonists HC-030031 or A-967079 at indicated concentrations for 24 hours. Thereafter, total RNA was extracted, and RT-qPCR of MX1 , OAS2, and DDX58 was carried out against RPL30 as an endogenous control gene. Individual values and mean ± SD are presented, n = 5–6. Control was set as 1, and the other values are given in relation to that value. Statistical analysis was performed using one-way ANOVA with Holm- Šídák post-test, and comparisons were carried out against the IFN-treated condition (second bar from the left). ∗, ∗∗, ∗∗∗, and ∗∗∗∗ denote P < .05, <.01, <.001, or <.0001, respectively.

Article Snippet: The following primary antibodies diluted 1:1000 in 5% BSA were used: OAS2 (E2G4K) Rabbit mAb #24344, MX1 (D3W7I) Rabbit mAb #37849, Rig-I (D33H10) Rabbit mAb #4200, Phospho-Stat1 (Tyr701) (D4A7) Rabbit mAb #7649, and Stat1 antibody #9172 (all from Cell Signaling Technology, CST).

Techniques: Expressing, Cell Culture, Quantitative RT-PCR, Control

Journal: Molecular Pharmacology

Article Title: Transient receptor potential ankyrin 1 promotes the expression of interferon-stimulated antiviral genes in human A549 lung epithelial cells

doi: 10.1016/j.molpha.2025.100098

Figure Lengend Snippet: The effect of the TRPA1 antagonists on the protein expression of the IFN-stimulated antiviral genes MX1, OAS2 . and DDX58 . A549 cells were cultured with IFN beta (10 ng/mL; A, C) or IFN alfa-2a (10 ng/mL; B, D) for 24 hours with or without the TRPA1 antagonists HC-030031 (HC; 100 μ M) or A-967079 (A96; 100 μ M), total protein was extracted, and indicated proteins were detected by western blotting. In panels A and B, representative blots of the indicated proteins (with 2 biological replicates of each treatment) are shown following IFN beta (A) and IFN alfa-2a (B) treatment, respectively. Actin was detected as a loading control. Densitometric analysis is shown in panels C and D. Individual values and mean ± SD are presented, n = 5–6. IFN-treated conditions (second bar from the left) were set as 1, and the other values are given in relation to and statistically tested against that value. Statistical analysis was performed using one-way ANOVA with Holm- Šídák post-test. ∗, ∗∗, ∗∗∗, and ∗∗∗∗ denote P < .05, <.01, <.001, or <.0001, respectively.

Article Snippet: The following primary antibodies diluted 1:1000 in 5% BSA were used: OAS2 (E2G4K) Rabbit mAb #24344, MX1 (D3W7I) Rabbit mAb #37849, Rig-I (D33H10) Rabbit mAb #4200, Phospho-Stat1 (Tyr701) (D4A7) Rabbit mAb #7649, and Stat1 antibody #9172 (all from Cell Signaling Technology, CST).

Techniques: Expressing, Cell Culture, Western Blot, Control

Journal: Molecular Biomedicine

Article Title: Activated oligoadenylate synthetase-ribonuclease L pathway promotes endothelial pyroptosis and impairs diabetic wound healing via thioredoxin-interacting protein m 6 A methylation

doi: 10.1186/s43556-025-00399-9

Figure Lengend Snippet: Elevated expression of OSA-RNase L in the HUVECs stimulated by high glucose. Immunofluorescence assay and semi-quantitative analysis of dsRNA ( a - b ). dot blot assay of dsRNA in the diabetic mouse skin ( c ). RT-qPCR assay of OAS1 mRNA ( d ), OAS2 mRNA ( e ), OAS3 mRNA ( f ), RNase L mRNA ( g ) expression in HUVECs stimulated by high glucose. Western blot assay of OAS1, OAS2, OAS3 and RNase L protein ( h ), and immunofluorescence assay and semi-quantitative analysis of OAS1, OAS2, OAS3 and RNase L (i-p). The results are presented as the mean ± SEM, * p < 0.05, ** p < 0.01, **** p < 0.0001. Each experiment was replicated for thrice. The number of mice used in this experiment was four per group

Article Snippet: The membranes were incubated overnight at 4 °C with specific primary antibodies: OAS1 (14955-1-AP), OAS2 (19279-1-AP), OAS3 (21915-1-AP), RNase L (22577-1-AP), NLRP3 (19771-1-AP), GSDMD (20770-1-AP), IL-1β (16806-1-AP), IL-18 (10663-1-AP), METTL3 (15073-1-AP), TXNIP (18243-1-AP), and Tubulin (10094-1-AP), all from Proteintech (Wuhan, China).

Techniques: Expressing, Immunofluorescence, Dot Blot, Quantitative RT-PCR, Western Blot